Figure 5.

Western blot analyses demonstrating limited release of MUC20 by the ocular surface epithelia. (A) Low levels of MUC20 were detected in the cell culture media of stratified HCLE and HCjE cells using an antibody targeting the N-terminal domain of the protein. A total of 40 μg cell lysates (CL) was used as positive control. Experiments were performed independently in triplicate. (B) The MUC20 was not detected in human tear washes pooled from three normal human subjects using antibodies recognizing the N- and C-terminal domains. On the other hand, MUC16 was present in the tear washes, as reported previously.²⁹