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. 2014 May 27;23(20):5418–5428. doi: 10.1093/hmg/ddu265

Figure 1.

Figure 1.

Phosphorylation of AKT/mTOR pathway proteins in adrenals of WT and AdKO mice. (A) Phosphorylated S6K1, rpS6, 4E-BP1 and AKT were detected in adrenal tissues from 12 to 14 month-old WT and AdKO mice. (B) Phosphorylation signal illustrated in A was quantified in five to six samples (values ± SEM). Values represent relative band density of the phosphorylated forms over total signal of the corresponding proteins, expressed as a percentage of the mean of WT. *P < 0.05. (C) Immunostaining for P-rpS6 in adrenal sections of vehicle-treated (Vhl) WT (a) and AdKO (b) compared with rapamycin-treated WT (c) and AdKO (d) mice. Dotted line delineates medullary (M) and cortical compartments, normal cortex (double headed arrow) and tumoral inner cortex (double dotted arrow) are indicated. Scale bars, 100 µm.