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. 2014 May 27;23(20):5418–5428. doi: 10.1093/hmg/ddu265

Figure 6.

Figure 6.

Ser112 and Ser136 phosphorylation of BAD in adrenals from WT and AdKO mice. (A) P-rpS6, BAD, P-BAD Ser112 and P-BAD Ser136 were detected in adrenal tissues from ACTH-treated or from vehicle-treated WT mice (Vhl). (B) Results illustrated in A were quantified (values ± SEM). Values represent relative band density over actin expressed as a percentage of the mean of vehicle-treated mice for P-rpS6 and BAD, and relative band density over BAD expressed as a percentage of the mean of vehicle-treated mice for the two forms of P-BAD. n = 7. (C) BCL-XL, BAD, P-BAD Ser112 and P-BAD Ser136 were detected in adrenal tissues from 12 to 14 month-old WT and AdKO mice. (D) Results illustrated in C were quantified on five WT and six AdKO mice (values ± SEM). Values represent relative band density over actin expressed as a percentage of the mean of WT mice for BCL-XL and BAD, and relative band density over BAD expressed as a percentage of the mean of WT mice for the two forms of P-BAD. (E–G) P-BAD Ser112, P-BAD Ser136, P-rpS6 and P-AKT were detected in PPNAD human adrenal samples from patients carrying germline PRKAR1A-inactivating mutation (19) and counterstained with hematoxylin. *P < 0.05. Scale bars, 100 µm in magnification and 500 µm in entire adrenal.