Table 1. Total Number of Identified Peptides and Glycopeptides from LC–MS Data Sets Using GlycReSoft (MS Only)a.
| total no. of nonglycosylated peptides identified (MS1 only) |
no. of
ambiguous matches (more than one match) |
total no. of glycopeptides identified (MS1 only) |
total
% relative abundance of glycopeptides |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| glycoprotein | no. of theoretical peptides + glycopeptides | HILIC-C18 | C18 | no. of theoretical glycopeptides (search space) | HILIC-C18 | C18 | HILIC-C18 | C18 | HILIC-C18 | C18 |
| transferrin | 1576 | 2 | 77 | 1230 | 6 | 5 | 54 | 38 | 97.67% | 0.86% |
| AGP | 4009 | 2 | 19 | 3936 | 16 | 23 | 155 | 141 | 99.93% | 3.40% |
| hemagglutinin | 57 358 | 4 | 39 | 56 886 | 725 | 278 | 1220 | 530 | 99.97% | 2.52% |
a
Only the glycopeptides/peptides found in all three replicate data were included. Relative abundances were calculated as a percentage of total abundances of all identified compounds. Note that deamidation was considered as a variable modification only in the case of HA.