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. 2014 Sep 3;79(19):9170–9178. doi: 10.1021/jo501529k

Figure 2.

Figure 2

TLC plate showing the different products following 1 h incubations with MEP, EP, MTP, or EEP (500 μM) with IspDF (4.8 μM) and [α-32P]CTP (150 μM, 40 μCi/μmol) or IspDF, IspE (6.2 μM), [γ-32P]ATP (150 μM, 320 μCi/μmol), and [α-32P]CTP (150 μM, 40 μCi/μmol). The reactions were quenched with methanol. Lane 1: MEP, CTP (control); lane 2: MEP, ATP (control); lane 3: MEP, IspDF, CTP; lane 4: MEP, IspDF, IspE, ATP, CTP; lane 5: EP, IspDF, CTP; lane 6: EP, IspDF, IspE, ATP, CTP; lane 7: MTP, IspDF, CTP; lane 8: MTP, IspDF, IspE, ATP, CTP; lane 9: EEP, IspDF, CTP; lane 10: EEP, IspDF, IspE, ATP, CTP.