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TLC plate showing the different products formed following a 1 h incubation of MESP (500 μM), IspDF (4.8 μM), or [H281S]IspDF, IspE (6.2 μM), unlabeled ATP or [γ-32P]ATP (150 μM, 320 μCi/μmol), and [α-32P]CTP (150 μM, 40 μCi/μmol). The reactions were quenched with methanol. Lane 1: MESP, [32P]CTP, [32P]ATP, IspDF, IspE; lane 2: MESP, [32P]CTP, [32P]ATP, [H281S]IspDF; lane 3: MESP, [32P]CTP, ATP, IspDF, IspE.
