Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jul 28;1(8):570–588. doi: 10.1002/acn3.88

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 The Authors. Annals of Clinical and Translational Neurology published by Wiley Periodicals, Inc on behalf of American Neurological Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

MCMV infection inhibits the proliferation of NSPCs via both direct and indirect mechanisms. (A–C) Mock- and MCMV-infected pregnant mice were injected with BrdU at 3 and 5 dpi, and the brains of their fetuses were removed at 3 h after the injection. (A) Detection of MCMV-IE1 Ag (green), BrdU (red), and SOX2 (magenta) by immunofluorescence staining of section of the MCMV-infected fetal cerebrum at 3 dpi. Nuclei were stained with DAPI (blue). At 3 dpi, BrdU incorporation is completely inhibited in MCMV-infected NSPCs of the VZ/SVZ between dotted lines. Scale bar = 20 μm. (B) The number of BrdU⁺ NSPCs adjacent to MCMV infectious focus in the MCMV-infected cerebrum (right) decreased in comparison with that in the mock-infected cerebrum (left) at 5 dpi (E18.5). Scale bar = 50 μm. (C) The number of BrdU⁺ cells in the uninfected areas of the VZ/SVZ in the mock- or MCMV-infected cerebrum at 3 or 5 dpi. Data are expressed as mean ± SEM of three fetuses. *P < 0.05 compared with mock-infected fetuses. (D–E) Mock- and MCMV-infected pregnant mice were injected with BrdU at 24 h after infection and then the fetuses were removed at 96 h after BrdU injection (E18.5, 5 dpi). (D) The number of BrdU⁺ cells in the cortical plate of the MCMV-infected cerebrum (right) decreased in comparison with the mock-infected cerebrum (left). Scale bar = 50 μm. (E) The number of BrdU⁺ cells in the upper layer or the lower layer of the cortical plate in the mock-infected or MCMV-infected cerebrum at 5 dpi. Data are expressed as mean ± SEM of three fetuses. *P < 0.05 compared with mock-infected fetuses. MCMV, murine cytomegalovirus; NSPC, neural stem/progenitor cells; VZ, ventricular zone; SVZ, subventricular zone.