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Immunoblots of the conditioned media (10 μL per track migrated in a 10% gel) of HEK 293a cells expressing either s-EGFP (transient transfection, 24-h collection) or PTH1-34-EGFP (stable transfectant, 96-h collection). The conditioned media were produced in the presence or absence of 10% fetal bovine serum, as indicated. Results representative of three experiments. Inset: Green epifluorescence of the stable transfectant cell line (400×).
