Figure 7.

Colocalization studies in HEK 293a cells coexpressing PTH₁R and CherryFP-conjugated Rab construction. (A) GTP-locked Rab5 colocalization. Cells were stimulated with the CM containing PTH_1-34-EGFP for 30 min; control cells were used to evaluate the green autofluorescence and Rab5 distribution under the same conditions. (B) Rab7 colocalization studies for longer incubation periods (as indicated). The colocalization is very rare at 3 h, but significant at 6 h (arrowheads). Epifluorescence, original magnification: 1000×.