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Plots showing 2-sec-butyl thiazole (SBT) binding to darcin and MUP11 in 25 mM PO4 3−, 25 mM NaCl, 298 K curve fit to a one-site model. (A) Darcin binds SBT with N (stoichiometry ratio) = 1.0, KD∼0.173 µM, ΔH = ∼ -13.1 kcal/mol and TΔS = ∼3.9 kcal/mol. (B) MUP11 binds SBT with N (stoichiometry ratio) = 1.0, KD ∼2.76 µM, ΔH ∼-9.8 kcal/mol and TΔS = ∼ 2.2 kcal/mol.
