| Cell Culture Reagents |
| Phosphate buffered saline (PBS) pH 7.2 - 7.6 |
| Dulbecco's Modified Eagles Medium (DMEM) |
| Fetal calf serum (FCS) filter sterilized |
| Penicillin/streptomycin |
| L-glutamine |
| 0.05% Trypsin-EDTA (1%) |
| 70% ethanol |
| Actinomycin D (1 μg/μl stock) |
| Cell Culture Equipment |
| Sterile Pasteur Pipettes |
| A range of cell culture-treated plastic ware suitable for the maintenance and treatment of cells in culture |
| Water bath set at 37 °C |
| Laminar air flow hood |
| Incubator set at 37 °C, 5% CO2 |
| Hypoxia Workstation set at 37 °C, 5% CO2, 1% O2 |
| Glass coverslips |
| precision forceps |
| Hemocytometer |
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