Figure 3. Phylogenetic tree showing the relationship of the twenty-eight retrieved bands from DGGE profile, on the basis of mglA gene fragment sequences obtained.

The numbers of the sequences in this tree (e.g., B1) refer to the numbers in the denaturing gradient gel (i.e., 1 [Fig. 2B]). The tree was constructed using the neighbor-joining method and analysis was based on 194 nucleotides. Numerals at nodes indicate bootstrap values derived from 1000 replications [29].