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. 2014 Oct 3;9(10):e108997. doi: 10.1371/journal.pone.0108997

Figure 3. Rat GRP78 mRNA, rno-miR-376a expression in primary rat granulosa cells induced by FSH.

Figure 3

Primary rat granulosa cells were prepared, and the indicated reagents were added to the medium after 24 h of culture. Cells were then incubated with FSH (30 ng/mL) and estradiol (10 nM) for 48 h in the same way as described in Fig. 2. The time after 48 h of incubation with FSH and estradiol was considered “0 h.” Total RNA was isolated, and rno-miR-376a expression levels was determined using real-time RT-PCR at the indicated time. The amounts of rno-miR-376a in the 0 h group were set at 1. Data were normalized for 4.5S RNA(H) levels in each sample and represent the mean ±SE of 3 independent experiments.