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. 2014 Oct 3;9(10):e108997. doi: 10.1371/journal.pone.0108997

Figure 4. Effects of Pre-miR-376a (precursor) and Anti-miR-376a (inhibitor) transfection on rat GRP78 mRNA expression in primary rat granulosa cells.

Figure 4

Primary rat granulosa cells were prepared, and the indicated reagents were added to the medium after 24 h of culture. The cells were incubated with FSH (30 ng/mL) and estradiol (10 nM) for 48 h. Pre-miR-376a (precursor) or Anti-miR-376a (inhibitor) was transfected into the cells, and 30 ng/mL hCG was added 12 h later. The effects of precursor and inhibitor on the expression of GRP78 mRNA were measured using real-time RT-PCR, as described in the Materials and Methods. The expression of GRP78 mRNA at hCG 0 h in the control was set at 1. Each value represents the mean ±SE of three independent experiments.