Figure 2. SCF, FL and TPO-dependent growth is impaired in CALM ^−/− LSK cells.

(A) (B) (C) LSK cells isolated from fetal liver of WT, CALM ^+/−, and CALM ^−/− mice were cultured in αMEM supplemented with 10% FBS in the presence of the indicated cytokines (100 ng/ml each). Total viable cell number was evaluated by the Cell Titer Glo Reagent (Promega) Data are shown as the mean ± SD of three cultures (n = 3, *p<0.05). SCF, stem cell factor; FL, Flt-3 ligand; TPO, thrombopoetin.