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. 2014 Oct 3;9(10):e109441. doi: 10.1371/journal.pone.0109441

Figure 3. Endocytosis of SCF-bounded KIT isn’t affected by CALM deficiency.

Figure 3

(A) WT and CALM−/− murine embryonic fibroblasts (MEFs) engineered to express KIT were incubated with biotinylated SCF for 60 min, and then with the APC-conjugated streptavidin for 30 min at 4°C. After stripping unincorporated SCF, the amount of the internalized SCF-KIT complex was quantified from the fluorescence intensity at the indicated times. (B) Uptake of SCF in WT or CALM −/− MEFs. The vertical axis indicates the ratio of mean fluorescence intensity, MFI (internalized SCF-KIT complex/initial surface KIT) (Data represent means ± SD, n  = 3, n.s.: not significant (p = 0.079)).