Figure 6. SCF-induced KIT trafficking from early to late endosome is impaired in CALM^−/− MEFs.

Whole cell lysates were prepared from WT and CALM^−/− MEFs after 15-min SCF stimulation. These lysates were fractionated with OptiPrep density gradient as described in Materials and Methods. Subcellualr localization of KIT was analyzed by immunoblot analysis with the anti-KIT Ab. EEA1 was utilized as an early endosome marker, and LAMP1 as a late endosome to lysosome marker.