Figure 2. Growth inhibition of E. faecalis by KGGK released from P(SA:RA) or from DLLA.

The side walls of 6 wells from line A of a 96 microwell plate were coated with the tested formulation (100 μg peptide+100 mg polymer, ratio 1∶1000). Fresh medium was added to the first line of wells and was transferred every 24 hrs to a new line below for a week. Then the bacteria were added to the tested wells and the plate was incubated at 37°C in a VERSAmax microplate reader and OD₆₅₀ in each well was followed automatically for 20 hrs. (A, C) KGGK+DLLA. (B, C) KGGK+ P(SA:RA) (D, E) weekly release of both formulations. Percent growth inhibition calculated compared with that of the non- treated bacteria during the logarithmic phase of the non treated bacteria. Generation time was calculated from each curve using the section representing the exponential growth phase (C, E).