Table 1. In this table primer pairs which have been used in addition to primers described by Bakonyi et al. [10] are listed (available as online appendix at: http://www.cdc.gov/ncidod/EID/vol12no04/05-1379_app.htm).
| Sequence 5′–3′ (F, forward primer; R, reverse primer) | Primer position (refers to the sequence VLG_07, FJ425721) | Length of the PCR product (nt) |
| AGCACGAAGATCTCGATGTC(F)* | 49–68 | 593 |
| GTGCACCARCAGTCRATGTC(R)* | 641–622 | |
| CCGCGGATTGTCCTTGATAG(F) | 129–148 | 766 |
| CACGACGCGTTGCATYGTGT(R) | 894–875 | |
| CAATCTGTTGTGGCTCTAGG(F) | 1681–1700 | 845 |
| TCCATCCAGGCTTCCACATC(R) | 2525–2506 | |
| GCCGGAGCGATTCCTGTTGA(F) | 1732–1751 | 1340 |
| AGCTTCCARGTGTCGTTGAG(R) | 3071–3052 | |
| TCCTTGCAGTTGGAGGAGTT(F) | 2387–2406 | 826 |
| CCTGGTCTCCTGTTGTGATT(R) | 3212–3193 | |
| GGCACGCACAACCACTGAGA(F) | 3327–3346 | 642 |
| AGCAGCGGCACCACCACATT(R) | 3968–3949 | |
| GGCCTGCTACAGAAGTGATG(F) | 4190–4209 | 552 |
| CCTTAGTGGTGTGCCACAGT(R) | 4741–4722 | |
| GGTCTGGCAGAACTTGACAT(F) | 4243–4262 | 431 |
| CCAAGCAGACCTCGAGTCAT(R) | 4673–4654 | |
| TGCTGAGATCACAGGCTCTA(F) | 4380–4399 | 1021 |
| GTGTGGAGACATCAGCCTAT(R) | 5400–5381 | |
| AGATTGAGGACGGCTGTGCT(F) | 5224–5243 | 638 |
| TTGCGGCTGTCGATCACTCT(R) | 5861–5842 | |
| ATAGGCTGATGTCTCCACAC(F) | 5381–5400 | 591 |
| TTCTTCCTATGCGTCCTCT(R) | 5971–5953 | |
| CAGAGGCTCGCATCATGCTA(F) | 6047–6066 | 795 |
| ACACAGCGAGCTGGTTGTCA(R) | 6841–6822 | |
| CCTGAGCGCGAGAAGGTGTA(F) | 6112–6131 | 828 |
| GTGTCCTAGCAGGCTGCTAA(R) | 6939–6920 | |
| CCTGAGAACAGCTGACTTAC(F) | 6189–6208 | 519 |
| GTGGCAGCTCCTAAGATTAC(R) | 6707–6688 | |
| TGTTGGATGGCTGAAGTCTC(F) | 6715–6734 | 789 |
| TGCTGCTGCTGTAGTCAGAA(R) | 7503–7484 | |
| GACTCTGACCGTGACTGTGA(F) | 7203–7222 | 715 |
| ATAGCACCAGCCGCCTCTAC(R) | 7917–7898 | |
| AGCGGAAGCTATGCGATCTG(F) | 7275–7294 | 847 |
| TTCTACCTCGGCACTTGACG(R) | 8121–8102 | |
| GGCCATTACTGAAGTTGACC(F) | 7740–7759 | 1061 |
| ACAGCCAGTTCGTGGTCTCA(R) | 8800–8781 | |
| ACCGTCCGTGTCTTGGAGAT(F) | 8131–8150 | 448 |
| TTCCGTGGTAGTTCCAGGT(R) | 8578–8560 | |
| AGCTGACCTCGAGAATGAAG(F) | 9288–9307 | 990 |
| CGGACCTGATTRATTGCTAC(R) | 10277–10258 | |
| TAGCGCGGTCCATCATCGAG(F) | 9348–9367 | 1633 |
| GCGCACTGTGCCGTGTGGCT(R) | 10980–10961 | |
| GCCACCGGAAGTTGAGTAGA(F)* | 10515–10534 | 466 |
| CTGGTTGTGCAGAGCAGAG(R)* | 10962–10944 | |
| GCTGCGAGGTGATCCACGTA(F) | 10579–10598 | 398 |
| ACTGTGCCGTGTGGCTGGTT(R) | 10976–10957 |
Primers marked with stars are suitable for detection of both WNV lineages 1 and 2.