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. 2014 Oct;4(10):a018507. doi: 10.1101/cshperspect.a018507

Figure 3.

Figure 3.

IFN-γ- and TLR-induced immune GTPases involved in autophagic trafficking of Mtb and antimicrobial cargo to lysosomes. (A) Deletion of the Irgm1 gene leads to profound TB susceptibility after aerogenic challenge (MacMicking et al. 2003). Scale bar, 1.0 cm. (B) Targeting of endogenous Irgm1 to mycobacterial phagosomes in IFN-γ-activated macrophages. TO-PRO-3 detects nuclear and bacterial DNA. Original inset for higher magnification shown. Scale bar, 2 µm. (C) Recruitment of the 65-kDa guanylate binding protein, Gbp7, to mycobacterial phagosomes in IFN-γ-activated macrophages. Scale bar, 5 µm. Gbp7 helps recruit p67phox to assemble the phagocyte oxidase complex in this location (Kim et al. 2011). (D) Live imaging of Gbp7 vesicle recruitment (green) to internalized mycobacteria (red) over a 30.8-min data collection period. Image J software reveals fluorescence convergence within the chosen inset area (Kim et al. 2011).