Figure 4.

Differential recruitment of effectors to “trapped” and “escaped” Mtb in immunologically activated macrophages. (Left) Delivery of phagosomal bacilli to autolysosomes after autophagic capture requires both immune and nonimmune GTPases. Their position in the pathway and corresponding interacting partners are color matched. (Right) Cytosolically exposed bacilli following permeabilization of the phagosomal membrane by ESX-1 releases DNA for detection by STING. This recruits TBK-1 and probably LC3C for Mtb capture. The E3 ligase PARKIN also ubiquitinates Mtb directly or its surrounding membrane remnants for detection by the autophagic pathway. A preferential requirement for K63 over K48 ubiquitin linkage is reported (Manzanillo et al. 2013).