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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Plant J. 2014 Sep 20;80(2):197–206. doi: 10.1111/tpj.12643

Figure 2. Characteristics of recombinant IRX10-L.

Figure 2

(a) RP-HPLC of the products formed by incubating IRX10-L (0.25 μg/ μL) with UDP-Xyl (2 mM), Xyl6-2AB (0.25 mM) and MgCl2 (1mM) in HEPES-HCl (75 mM) pH 6.8 for the indicated times.

(b) The time-dependent abundance distributions of oligosaccharide products generated as described in (a) and quantified by integration of the peaks in the RP-HPLC elution profiles. Data are averages of three reactions with purified protein from two biological replicates. Observed product distributions are shown for 24 hours (blue) and 48 hours (red) as fractional populations (dotted lines with data point symbols); theoretical abundances (solid lines) are Poisson probabilities calculated as described in Experimental Procedures.