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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Plant J. 2014 Sep 20;80(2):197–206. doi: 10.1111/tpj.12643

Figure 3. TBL29/ESK1 catalyzed acetylation of xylooligosaccharides using acetyl-CoA as the donor.

Figure 3

(a) In vitro O-acetyltransferase reaction scheme.

(b) MALDI-TOF MS of the products after incubating TBL29/ESK1 for 16 hours with Xyl6-2AB and acetyl-CoA. Each transfer of an O-acetyl (Ac) group increases the mass of the Xyl6-2AB acceptor by 42 Da to generate products corresponding to the annotated [M+H]+ ions.

(c) Amounts of acetylated xylosyl residues at O-2 (X2Ac) or O-3 (X3Ac) formed during the first 6 hours of the TBL29/ESK-catalyzed reaction. The product concentrations were determined by real time 1H NMR analysis.