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Since the commercially available IL11Rα antibodies are not sufficiently sensitive to detect endogenous IL11Rα protein in the MDA-MB-468 cells, we tested the ability of shRNA to down-regulate the expression of exogenously expressed IL11Rα. Cells overexpressing IL11Rα were stably transduced with IL11Rα-targeting shRNAs and the expression of IL11Rα and -actin (loading control) were analyzed by immunoblotting.
