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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Clin Cancer Res. 2014 Aug 12;20(19):5085–5096. doi: 10.1158/1078-0432.CCR-14-1038

Figure 1. Radiosensitization by combined Wee1 and PARP inhibition.

Figure 1

AsPC-1 (A) and MiaPaCa-2 (B) pancreatic cancer cell lines were treated with AZD1775 (100nM), olaparib (1μM), and radiation (0–6Gy) according to the schedule illustrated (D). At the end of treatment cells were processed for clonogenic survival. Data are from a single representative experiment (A–B) or are the mean radiation enhancement ratio (RER) ± SE for n = 3 independent experiments (C). Cytotoxicity in the absence of radiation treatment was calculated by normalizing the plating efficiencies of drug treated-cells to non-drug treated cells where plating efficiencies for non-drug treated AsPC-1 and MiaPaCa-2 cells were 0.2 and 0.3, respectively (C). Statistical significance (P<0.05) is indicated vs control*, AZD1775‡, and olaparib¥.