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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Clin Cancer Res. 2014 Aug 12;20(19):5085–5096. doi: 10.1158/1078-0432.CCR-14-1038

Figure 4. Combined inhibition of Wee1 and PARP causes persistent radiation-induced DNA damage.

Figure 4

AsPC-1 (A) and MiaPaCa-2 (B) cells were treated with AZD1775/olaparib for 1 hour pre- and for the indicated times post-RT. At each time point post-RT, cells were fixed for flow cytometric analysis and analyzed for γH2AX positivity and DNA content. Data shown are the mean percentage of γH2AX positive cells ± SE from n=3–4 independent experiments. Statistical significance (P<0.05) is indicated vs RT*, AZD1775+RT‡, and olaparib+RT¥.