Figure 7.

Chromatin Landscape of Naive Human Pluripotency

(A–E) ChIP-Seq tracks for H3K4me3 and H3K27me3 in WIBR2 human ESCs cultured in primed hESM (red) or naive 6i/L/A medium (P18) (blue) at five classes of genes: (A) developmental genes that are bivalent in the primed state and exhibit loss of H3K27me3 in the naive state; (B) naive pluripotency genes that are bivalent in the primed state and exhibit loss of H3K27me3 in the naive state; (C) naive pluripotency genes that acquire H3K4me3 in the naive state; (D) master transcription factors that have a signal for H3K4me3, but not H3K27me3, in both naive and primed states; and (E) genes that are strongly downregulated in the naive state and acquire increased H3K27me3 signal.

(F) ChIP-Seq analysis for H3K4me3 and H3K27me3 at Polycomb target genes in WIBR2 human ESCs cultured in primed medium (left) or naive 6i/L/A medium (right).

(G) Average H3K4me3 and H3K27me3 signal at Polycomb target genes in WIBR2 human ESCs cultured in primed medium (red) or naive 6i/L/A medium (blue).