Fig. 1.

Schematic diagram of the MRCA approach using a bootstrap sample. Additional sequences were aligned to the pre-existing FunTree alignment. This alignment was then used to build a maximum-likelihood tree, with 100 bootstrap replicates. The MRCA sequence was obtained from 98 of the trees in the bootstrap set. The 98 sequences were clustered at 60 % sequence identity. A representative from each cluster was submitted to the homology modelling server PHYRE2. Functional analysis was then carried out on each of these homology models compared to pre-constructed active site templates