Figure 2.

(A) Imaging positions for mice in the IVIS Spectrum system. Each mouse was imaged twice at each time point in two different positions, and results from the two images were averaged for each mouse/time point. (B) Regions of interest for quantifying fluorescent signals in each knee. The region of interest was a uniform circle of 12.3 mm² that was anatomically selected around the knee on a grayscale photograph of the mice, such that the selection criteria were unbiased by the fluorescent signals.