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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: J Immunol. 2014 Sep 8;193(8):3902–3913. doi: 10.4049/jimmunol.1302998

Figure 6. Flagellin post onset treatment in CIA contributes to joint inflammation, elevated joint myeloid cell trafficking and TNF-α production.

Figure 6

A. Changes in joint circumference was determined in CIA mice that were treated i.p. with PBS or flagellin (20 µg) on day 33, n= 10. B. Ankles were harvested on day 57 from CIA mice treated with PBS or flagellin and were H&E stained (original magnification x 200), n=7. C. STs from CIA mice treated with PBS or flagellin were harvested on day 57 and were immunostained with anti-F480 antibody (original magnification x 200), arrows demonstrate F480+ cells. D. Macrophage staining was quantified on a 0–5 scale, n=7. E. TNF-α protein levels (pg/ml) were quantified by ELISA in ankle homogenates from CIA mice treated with PBS or flagellin, n=7. F. Transcription of IFN-β was determined by real-time RT-PCR in CIA ankles which had received post onset treatment of flagellin or control and the data are shown as fold increase above PBS group and are normalized to GAPDH, n=5.Values are mean ± SE. * indicates p<0.05.