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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: J Immunol. 2014 Sep 8;193(8):3902–3913. doi: 10.4049/jimmunol.1302998

Figure 7. TLR5 ligation facilitates osteoclast formation in vivo in RA animal models.

Figure 7

A. Ankles harvested from CIA mice treated with PBS or flagellin were TRAP stained (TRAP+ cells are shown by arrows) and (B) the number of TRAP+ cells were counted per section, n=7. C. Transcription of calcitonin receptor (CTR), Cathepsin K (CTSK) and RANKL was determined by real-time RT-PCR in CIA ankles which had received post onset treatment of flagellin or control and the data are shown as fold increase above PBS group and are normalized to GAPDH, n=5. D. Changes in joint circumference in mice i.a. injected with PBS or 20µg of flagellin; n=5. Ankles harvested from local injection of PBS or flagellin on day 10 were H&E (E) and TRAP (F) stained (TRAP+ cells are shown by arrows) and (G) number of TRAP+ cells were counted per section, n=5. Values are mean ± SE. * indicates p<0.05.