Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primer sequences for qRT-PCR.
| Gene | Primer | Accession Number | Sequence | Amplicon Size |
|---|---|---|---|---|
| r28S | q-28S-Fwd | NR_003287.2 | CGAGATTCCCACTGTCCCTA | 158bp |
| q-28S-Rvs | GGGGCCTCCCACTTATTCTA | |||
| COL1A1 | q-COL1A1-Fwd | NM_000088 | TCTGCGACAACGGCAAGGTGT | 147bp |
| q-COL1A1-Rvs | CGACGCCGGTGGTTTCTTGGT | |||
| ACTA2 | q-ACTA2-Fwd | NM_001613.2 | CCAAGGCCAACCGGGAGAAAATGA | 97bp |
| q-ACTA2-Rvs | GCATAGAGAGACAGCACCGCCTGG | |||
| TIMP-I | q-TIMP-I-Fwd | NM_003254.2 | AATTCCGACCTCGTCATCAGG | 123bp |
| q-TIMP-I-Rvs | ATCCCCTAAGGCTTGGAACC |
