Figure 5. Dicer regulates Cd4 and Cd8 silencing and expression of Runx3 and Zbtb7b in positively-selected αβ T cells.

A, Representative CD4 and CD8 staining on CD24^loTCRβ^hi mature thymocytes of R1 OT-I and LBD R1 OT-I mice. B, Average percentages of CD4⁺CD8⁺, and CD4⁺CD8⁺ cells amongst CD24^loTCRβ^hi mature thymocytes of R1 OT-I and LBD R1 OT-I mice. C, Representative CD4 and CD8 staining on CD24^loTCRβ⁺ splenocytes of BD R1 OT-I and LBD R1 OT-I mice. D, Average percentages of CD4⁺CD8⁺, and CD4⁺CD8⁺ cells amongst CD24^loTCRβ⁺ splenocytes of R1 OT-I and LBD R1 OT-I mice. E, Representative CD4 and CD8 staining on CD24^loTCRβ^hi mature thymocytes of R1 OT-II and LBD R1 OT-II mice. F, Average percentages of CD4⁺CD8⁺, and CD4⁺CD8⁺ cells amongst CD24^loTCRβ^hi mature thymocytes of R1 OT-II and LBD R1 OT-II mice. G, Representative CD4 and CD8 staining on CD24^loTCRβ⁺ splenocytes of BD R1 OT-II and LBD R1 OT-II mice. H, Average percentages of CD4⁺CD8⁺, and CD4⁺CD8⁺ cells amongst CD24^loTCRβ⁺ splenocytes of R1 OT-II and LBD R1 OT-II mice. I, qRT-PCR for primary (un-spliced) Cd4 transcripts in sorted splenic populations from WTR1 OT-I, or LBD R1 OT-I mice. J, Runx3 Western blot in sorted splenic CD4⁺ or CD8⁺ cells from WT mice and CD8⁺ or CD4⁺CD8⁺ DP) cells from LBD R1 OT-I. Three independent replicates were performed; a representative blot is shown. K, Zbtb7b qRT-PCR in sorted populations from R1 OT-II or LBD R1 OT-II mice. B, D, F, H, I, and K, The numbers of mice analyzed are indicated. At least 3 independent experiments were performed in each case.