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. Author manuscript; available in PMC: 2014 Oct 5.
Published in final edited form as: Cell Metab. 2013 Sep 3;18(3):355–367. doi: 10.1016/j.cmet.2013.08.003

Figure 6. OPN Is Required for CLS Formation and ADRB3-Mediated Adipogenesis from PDGFRα+ Cells.

Figure 6

(A) Reduction in weight of gWAT from WT and Spp1-KO mice after 3 days of CL treatment (mean ± SEM; n = 4–6; **p < 0.01, ***p < 0.001).

(B) Reduction in size of gWAT adipocytes from WT and Spp1-KO mice after 3 days of CL treatment (mean ± SEM; n = 4–6; **p < 0.01).

(C) Immunohistochemical analysis of CLS in paraffin sections of gWAT from wild-type (WT) and Spp1-KO mice under control condition (CTL) or after 3 days of CL treatment (CLd3), stained for F4/80 (green).

(D) Quantitative PCR analysis of macrophage marker expression in gWAT from control and Spp1KO mice (mean ± SEM; n=10–11 from two independent cohorts; *p < 0.05, **p < 0.01, ***p < 0.001).

(E) FACS analysis of EdU incorporation in PDGFRα+ cells from gWAT of WT and Spp1-KO mice treated with CL for 3 days and injected with EdU 2 hr before analysis (mean ± SEM; n = 3–4; **p < 0.01).

(F) Representative images of paraffin sections of gWAT stained for PLIN1 and EdU and quantification of PLIN1+EdU+ cells (mean ± SEM; n = 4; **p < 0.01). Scale bars, 100 µm except high-magnification field in (F), where scale bar, 20 µm. See also Figure S6.