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. 2014 Aug 22;24(10):1201–1213. doi: 10.1038/cr.2014.113

Figure 2.

Figure 2

Transcriptional regulatory cascades revealed by deletion of Baf250a in SAN. (A) Protein level of Baf250a in the SAN after tamoxifen induction in Baf250af/f;Hcn4CreERT2 mice. Scale bar, 100 μm (top panel), and 25 μm (bottom panel). (B) Unbiased clustering of time course expression data. (C, D) qPCR validated relative transcript levels of representative genes in profiles 4 and 16 (n = 3 in each group). *P < 0.05. (E) Protein levels of Tbx3 after deletion of Baf250a in the SAN. Scale bar, 50 μm. (F) Enhanced expression of Nkx2.5 after deletion of Baf250a in SAN. Scale bar, 50 μm. (G) Direct binding of Baf250a to the promoter of Tbx3 as examined by ChIP-qPCR (n = 3 in each group). *P < 0.05. (H) Binding of Tbx3 to the promoters of Nkx2.5, Gata4, and Tbx5 as examined by ChIP-qPCR (n = 3 in each group).