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. 2014 Sep 5;24(10):1181–1200. doi: 10.1038/cr.2014.118

Figure 1.

Figure 1

The generation of stage-specific hESC reporter lines by labeling sequential developmental genes of β cells. (A) Schematic of a five-stage pancreatic endocrine differentiation protocol from hESCs represented by important marker genes. The genes labeled with eGFP are in green and those labeled with Tdtm are in red; unlabeled genes are in black. (B) The reporter cell lines constructed in this study. Dual-reporter cell lines were named with the second targeted gene locus followed by “DR” for example, the “FOXA2-DR” cell line stands for the “FOXA2-Tdtm/NGN3-eGFP dual-reporter” cell line. Abbreviations: DE (definitive endoderm); PG (primitive gut tube); PF (posterior foregut); PP (pancreatic progenitor); EP (endocrine progenitor); EC (endocrine cells); INS (INSULIN); GCG (GLUCAGON); Tdtm (TdTomato).