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. 2014 Sep 26;24(10):1231–1249. doi: 10.1038/cr.2014.127

Figure 3.

Figure 3

PHF2 was required for osteoblast differentiation. (A) Temporal changes in Runx2 and PHF2 levels in C2C12 cells treated with 100 ng/ml of BMP2 were analyzed by immunoblotting. (B, C) C2C12 stable cell lines expressing non-targeting (Sh-Con) or PHF2-targeting (Sh-PHF2) shRNA were incubated with BMP2 for the indicated days. C2C12 differentiation toward osteoblasts was monitored by staining ALP (B) and by measuring its activity (C). (D) C2C12 cells under BMP2-induced differentiation were stained with Alizarin. (E) The mRNA expression levels of osteogenic genes were analyzed by RT-qPCR. Each point represents the mean ± SD (n = 3) of relative values to day 0 and * denotes P < 0.05 versus the wild-type value.