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. 2014 Oct 6;4:6528. doi: 10.1038/srep06528

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(a) Typical two examples of the fluorescent intensity trace in each FliI-YFP spot in Salmonella MKM30 strain harboring pJSV203 under continuous TIRF illumination after one-shot photobleaching using a strong excitation laser (green band). Peaks labeled with numbers in red show intensity recovery of a single FliI-YFP molecule within a time period of 60 seconds. (b) The number of times of single FliI-YFP molecule turnover per minute. Each YFP spot was counted in Salmonella MKM30 (ΔfliI) strain harboring pJSV203 (as indicated as FliI-YFP, light blue) (n = 89) and pSY001 (as indicated as FliI(K188I)-YFP, red) (n = 112) and NH0027 (ΔflhA ΔfliI) carrying pJSV203 (as indicated as ΔflhA/FliI-YFP, green) (n = 102).