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. Author manuscript; available in PMC: 2014 Oct 6.
Published in final edited form as: Circ Res. 2014 Jan 14;114(6):947–956. doi: 10.1161/CIRCRESAHA.114.303312

Figure 5. Peptidylarginine deiminase (PAD) inhibition abrogates neutrophil extracellular trap (NET) formation and alters anti-NET autoantibody profiles in Apoe−/− mice.

Figure 5

High-fat chow-fed Apoe−/− mice were treated with vehicle or Cl-amidine from 7 to 18 weeks of age. A, Body weight was recorded at the indicated time points. B, Serum was collected at 18 weeks of age and total cholesterol, triglycerides, and HDL were determined by direct measurement; LDL was calculated. *P<0.05; no other comparison of vehicle versus Cl-amidine was significant C, Bone marrow neutrophils were isolated at 18 weeks of age and stimulated with phorbol-12-myristate-13-acetate (PMA). **P<0.01. D, Neutrophil-platelet aggregates (NPA) were determined in blood at 18 weeks of age. NPA were defined as events positive for both Ly-6G and CD61. The percentage is calculated relative to total Ly-6G-positive cells. E, Serum cytokine levels were measured by multiplex assay; ns=not significant. A-E, n=10 per group. For A, C, D, and E, the mean and SEM are plotted. For B, boxes represent the median, 25th percentile, and 75th percentile; whiskers delineate the minimum and maximum values.