Figure 7. Vaccination with the p846 inhibited M. bovis BCG replication after challenge. Four weeks after the last immunization, mice were intranasally infected with 1 × 107 CFU BCG. Six weeks post infection, the mice were sacrificed and the numbers of BCG in the lungs and spleens of mice were determined by in vitro colony formation assays. (A) The numbers of BCG in the lungs. (B) The numbers of BCG in the spleens. Data are presented as the mean ± SD (n = 6) and are one representative of three separate experiments. *P < 0.05, **P < 0.01, ***P < 0.001.