Table 1. Overview of the primers.

Protein and Primer name	Primer sequences	Restriction enzyme sites
Rv3615c	P1 5′-CGGCTAGCGC CACCATGACG GAAAACTTGA CCGTCCAG-3′	NheI
	P2 5′-CCCTCGAGTC ACTTATCGTC GTCATCCTTG TAATCGGTAA ACAACCCGTC GATAGC-3′	XhoI
	P3 5′-TAGTTGTACA TGATTTGCGA GGTAAACAAC CCGTCGATAG CCTT-3′
Mtb10.4	P4 5′-CGGCTAGCGC CACCATGTCG CAAATCATGT ACAACTACCC C-3′	NheI
	P5 5′-CCCTCGAGTT ACTTATCGTC GTCATCCTTG TAATCGCCGC CCCATTTGGC GGCT-3′	XhoI
	P6 5′-TCGCAAATCA TGTACAACTA CCCCG -3′
	P7 5′-CTGGCCTGTT GCTGCAAGCG CCTGGTCGAC GCCCGCTATC ACGCCGCCCC ATTTGGCGG-3′
Rv2660c	P8 5′-CGGCTAGCGC CACCGTGATA GCGGGCGTCG ACCAG-3′	NheI
	P9 5′-CCCTCGAGTT ACTTATCGTC GTCATCCTTG TAATCGTGAA ACTGGTTCAA TCCCAGTATC GC-3′	XhoI
	P10 5′-CTTGCAGCAA CAGGCCAGGC AAGCCA-3′
	P11 5′-CGCTCGAGTT ACTTATCGTC GTCATCCTTG TAATCGTGAA ACTGGTTCAA TCCCAGTATC GCGCA-3′	XhoI

Restriction enzyme sites in the 5′ terminal of primers were in bold and italics.