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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Microcirculation. 2014 Feb;21(2):159–170. doi: 10.1111/micc.12102

Figure 5. Matrigel plug neovasculature 3D modeling and analysis by X-ray μCT scanning in WT mice.

Figure 5

(A) Macroscopic appearance of Matrigel plugs with or without VEGF at 3 d (left), 7 d (middle), and 10 d (right). Bar, 10 mm. (B) Images of 3D models of neovasculature in Matrigel plugs (top, +VEGF; bottom, −VEGF; left, 7 d; right, 10 d) acquired by X-ray μCT scanning at 3 μm voxel resolution and 3D reconstruction. Bar, 10 mm. (C) Total vessel volumes in 3D reconstructed images from Matrigel plugs derived in WT mice. n=3, * p < 0.001. (D) Frequency distribution of the vascular diameters in Matrigel plugs at 7 d (red lines) and 10 d (black lines). Dotted lines, −VEGF; solid lines, +VEGF. (E) Individual vessel volumes plotted against vascular diameters in Matrigel plugs from (D) (* p < 0.001, ** < 0.0001).