Figure 1. A: Reduced Coomassie Blue stained SDS-PAGE analysis of samples collected at different time points following the previously published (original) fermentation process. Lane 1: Mark12™. Unstained Standard, lane 2: pre-induction; lane 3: 24 h induction; lane 4: 48 h induction; lane 5: 65 h induction; lane 6: post-centrifugation (harvest). B: Reduced Coomassie Blue stained SDS-PAGE analysis of samples collected at different time points following the optimized fermentation process. Lane 1: SeeBlue® Plus2 Pre-stained Protein Standard; lane 2: pre-induction; lane 3: 22 h induction; lane 4: 46 h induction; lane 5: 66 h induction; lane 6: 89 h induction; lane 7: 89 h induction post filter (clarified).