Table 2. Comparisons of the yield and purity of Na-GST-1 at each step of the original and optimized purification process.
Step | Process | Purity (%) | Recovery Yield (%) |
---|---|---|---|
QXL | Goud 2012 | 79 | 86 |
Original | 81 | 83 | |
Optimized | 91 | 75* | |
Butyl | Goud 2012 | 94 | 64 |
Original | 95 | 71 | |
Optimized | >96 | 84* | |
SEC | Goud 2012 | 98 | 94 |
Original | 99 | 98** | |
Optimized | >98 | 94* |
A “*” indicates that the calculation is based on SDS-PAGE and “**” indicates value is based on A280 measurement.