Table 3A. Yield Comparisons from triplicate processes (mg).
Step | Run 1 | Run 2 | Run 3 | Average | SD | CV |
---|---|---|---|---|---|---|
FS | 897 | 813 | 808 | 839 | 50 | 6.0% |
TFF | 852 (95%) |
772 (95%) |
768 (95%) |
797 (95%) |
47 (0) |
5.9% (0) |
QXL | 652 (73%) |
584 (72%) |
544 (67%) |
593 (71%) |
55 (3.2) |
9.2% 4.3% |
Butyl | 545 (61%) |
494 (61%) |
444 (55/%) |
494 (59%) |
51 (4.6) |
10.2% (4.9%) |
SEC75 | 527 (59%) |
438 (54%) |
431 (53%) |
465 (55%) |
54 (3.2) |
11.5% (5.8) |
(A) Comparison of the amount of Na-GST-1 in mg/mL obtained from the 3 purification runs. Single yields for each run as well as the average, the standard deviation, the SD, and the coefficient of variation between each run, CV, are reported. The cumulative % of recovery for along the process is presented in brackets.