Figure 1. Proliferation of total nucleated cells (TNC) after treatment with MENK. Isolated TNC were cultured with MENK for 7 d in vitro and cell numbers were measured by FCM. The TNC grew into distinguished percentage as compared with that in control. We have proved there were more opioid receptors on T cell in our earlier publications and this was directly responsible for the proliferation of T cells because MENK binded to the receptors, triggering the response.