Liver IRI triggers cAMP-PKA activation. Liver samples were harvested from B6 mice that were either sham-operated or subjected to 90min of partial liver warm ischemia, followed by various lengths of reperfusion. (a) cAMP; and (b) PKA activity levels were measured.
Distinct effects of activation vs. inhibition of cAMP-PKA pathway in liver IRI (6h of reperfusion after 90min of ischemia). (c) cAMP; and (d) PKA activity levels were assessed after Forskolin (activator), H-89 (inhibitor), or DMSO (control) treatment.
The hepatocellular function, as analyzed by (e) sALT levels, and (f) liver histology (representative H&E staining; magnification x100 and x400) of the ischemic liver lobes (**p<0.01, *p<0.001, n=6-8/group).