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. 2014 Oct 6;8:188. doi: 10.3389/fnsys.2014.00188

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Copyright © 2014 Mellott, Bickford and Schofield.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Photomicrographs showing examples of GAD-negative and GAD+ cells in the nucleus of the brachium of the inferior colliculus that were retrogradely labeled by a large injection of FluoroGold (FG) into the ipsilateral medial geniculate nucleus. For each panel, the upper image shows cells labeled with FG and the lower image shows the same region visualized for GAD immunoreactivity. (A,B) Examples of FG-labeled cells (open arrows) that were GAD-negative. In both panels, nearby cells were robustly labeled for GAD (blue cells). (C–F) Examples of FG-labeled cells (solid arrows) that were also GAD+. Scale bar = 20 μm. A,C–E: GP636; B: GP721.