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. 2014 Oct 6;8:188. doi: 10.3389/fnsys.2014.00188

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Copyright © 2014 Mellott, Bickford and Schofield.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Electron micrographs showing FR-labeled cortical boutons forming synapses in the ipsilateral nucleus of the brachium of the inferior colliculus (NBIC). (A–D) Cortical boutons (^*) are filled with DAB precipitate resulting from transport of FluoroRuby. Internal structures in the boutons were obscured by the DAB precipitate, but round synaptic vesicles could often appear negatively stained within the profiles. Postsynaptic densities were typically prominent (located between the white arrowheads). The postsynaptic targets of these cortical boutons were dendrites (green shading). Post-embedding immunogold staining was used to identify GABAergic profiles, indicated by gold particles (small black dots) (G+). GP363. Scale bar = 1 μm.