| Component | Volume | Final Concentration |
| BAC 2002-D6 at 5 ng/μl | 4 μl | -- |
| 5X Phusion GC Buffer | 20 μl | 1X |
| DMSO | 6 μl | 6% |
| dNTPs (10 mm) | 2 μl | 200 μM |
| Forward Primer (100 μM) | 1 μl | 10 μM |
| Reverse Primer (100 μM) | 1 μl | 10 μM |
| ddH2O | 65 μl | -- |
| Phusion DNA Polymerase | 1 μl | -- |
| Total: 100 μl |
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